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Advisory Committee on Health Effects of Endocrine Disruptors
The Supplement II to the Intermediary Report
1.2.3.2 |
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(ii) Quantitative analysis: Inject an appropriate amount of the
sample solution into the GC/MS. Check the peak areas for each
PAE divided by the peak area of the internal standard against
the calibration curve to obtain the concentrations of the PAEs.
Use the total area of the two major peaks for DiOP and of the
five major peaks for DiNP.
(iii) Calibration curve: Construct the calibration curve using
the PAE peak areas, obtained by injecting an appropriate amount
of the standard PAE solutions containing the internal standard,
divided by the peak area for the internal standard. Use the
total area of the two major peaks for DiOP and of the five major
peaks for DiNP.
[Detection and determination limits]
Detection limits are set to 3 ng/ ml for DBP, BBP and DEHP (the
DBP and DEHP levels in the operation blank test can be reduced
as described below) and 20 ng/ml for DiOP and DiNP.
Determination limits are 10 ng/ml for DBP, BBP and DEHP, and 50
ng/ml for DiOP and DiNP.
[Notes]
1 PAEs are used as plasticizers in PVC resin. PAEs have
attracted attention because PVC are widely used in home
appliances and building materials, from which PEAs may be
released. This means that the blank value of environmental
origin can be problematic in PAE analysis, and should therefore
be reduced as much as possible.
The items listed below are known to be effective in reducing the
operation blank of DBP and DEHP from the usual order of 100 ng/ml
to less than 10 ng/ml.
a) Use distilled water washed with n-hexane of the PAE analysis
grade.
b) Heat to 200℃ for 2 h and let stand to cool all the tools
except one-mark pipettes and measuring flask used in the
analysis session, including the trap for the evaporator, and
wash with n-hexane of the PAE analysis grade immediately before
use. Use tweezers when washing the tools. Never touch them with
the hand.
c) Wash the hands thoroughly with soap and water before
operation. Avoid touching objects other than the analytical
tools or instruments. In particular, be sure to wash the hands
every time touching the door knob.
d) Cover the bench with aluminum foil, which must be replaced
everyday.
e) Clean the evaporator by distilling acetone of the PAE
analysis grade to dryness immediately before use.
f) Handle the standard chemicals on a bench other than that for
analysis.
g) Never let the pipette tips touch other tools, instruments,
etc.
h) Handle the one-mark pipettes in the standard way; never use
the mouth to drive the liquid off.
i) Take the watch off before operation.
j) Keep the hair tidy.
k) Keep the nails short. |
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