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2 This method uses surrogates as the internal standards
for collection of the recovery ratio. The average recovery ratio
is 98.7-123.7% and relative standard deviation is 0.86-7.52%
when the surrogates are added to serum so that the concentration
of DBP, BBP and DEHP is 100 ng/ml each and that of DiOP and DiNP
200 ng/ml each.
3 Do not use long after the first opening of the package
because of possible contamination by PAEs from the environment.
4 If the water used gives interfering peaks, repeat
washing with n-hexane.
5 Always perform blank test to determine the quantity of
PAE that enters the analytical system during the session,
because contamination from the environment can be problematic in
PAE analysis.
6 It is recommended to check the performance of the GC/MS
by injecting only n-hexane a few times before measurements of
standards and samples.
7 E.g. Agilent technologies' HP-5MS and HP-5MS SV, or J&W
Scientific's DB-5MS.
[Literature]
Selected recent papers on analysis of PAEs in biological samples
are listed below.1) Inoue K, Kawaguchi M, Okada F, Yoshimura
Y, Nakazawa H, Column-switching high-performance liquid
chromatography electrospray mass spectrometry coupled with
on-line of extraction for the determination of mono- and di-(2-ethylhexyl)
phthalate in blood samples. Anal Bioanal Chem, 375, 527-33
(2003)
2) Takatori S, Kitagawa Y, Kitagawa M, Nakazawa H, Hori S,
Determination of di(2-ethylhexyl)phthalate and
mono(2-ethylhexyl)phthalate in human serum using liquid
chromatography-tandem mass spectrometry. J Chromatogr B, 804,
397-401 (2004)
3）Colon I, Dimandja JM, High-throughput analysis of phthalate
esters in human serum by direct immersion SPME followed by
isotope dilution-fast GC/MS. Anal Bioanal Chem, 380, 275-283
(2004)
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