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1. Introduction
Bisphenol A used as a resin monomer, phthalic acid esters used
as plasticizers, and alkylphenols used for manufacture of
surfactants are attracting public attention for their high
exposure levels. Risk analysis for these substances needs the
estimation of exposure to humans in addition to the evaluation
of effects on organisms.
For this purpose, a high-sensitivity measurement method of human
samples and basic studies on acquisition and storage of samples
are needed. As in other microanalytical processes, contamination
of the sample before the measurement must be prevented.
For the three analytes mentioned above existing in biological
samples, a high-sensitivity analytical procedure was developed,
and a guideline for analysis of endocrine disruptors in
biological samples was established.
While the biological effects of endocrine disruptors are
generally evaluated by in vivo experiments on animals, it has
been pointed out that effects on animals of exposure to
chemicals via the raising and experimental environment (feed,
litter, water bottle, air, etc.) should be understood for
reliable estimation of the low-dose effects.
Accordingly an analytical method was developed for determining
phytoestrogens, in addition to the abovementioned three
substances, in animal feeds and other materials in the
environment. Its application to actual samples has started.
2. Guideline for determination of endocrine disruptors in
foods
The temporary guideline prepared by the then Sampling and
Analysis Working Group was published in the Supplement to the
Interim Report (December 2001).
No information that would necessitate revision of the guideline
has since been found. |
