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(ii) Qualitative and quantitative analysis
Confirm that the SIM peak (m/z = 219) permits separation of the
base line, and use the area of the peak for quantitation using
the internal standard. Construct a calibration curve over a
concentration range of 5 to 100 ppb. Perform at least five
measurements of a standard solution of the concentration at the
lower end of the calibration curve. The standard deviation of
the results multiplied by 3 is the detection limit, and by 10
the determination limit. If operation blank tests are performed,
use the standard deviation of the results for the standard
solution or for the blanks, whichever the greater11.
[Literature on the procedure]
1. K. Inoue, Y. Yoshimura, T. Makino, H. Nakazawa: Determination
of
4-nonylphenol and 4-octylphenol in human blood samples by
high-performance liquid chromatography with multi-electrode
electrochemical coulometric-array detection. Analyst 125,
1959-1961 (2000)
2. K. Inoue, M. Kawaguchi, F. Okada, N. Takai, Y. Yoshimura, M.
Horie, S. Izumi, T. Makino, H. Nakazawa: Measurement of
4-nonylphenol and 4-octylphenol in human urine by
column-switching liquid chromatography-mass spectrometry.
Analytica Chimica Acta 486, 41-50 (2003)
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