Advisory Committee on Health Effects of Endocrine Disruptors
The Supplement II to the Intermediary Report
1.4.1.3

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3.1 Bisphenol A

• High-sensitivity column switching HPLC-fluorescence photometry: detection limit 0.04 ppb for serum and peritoneal fluid, 0.20 ppb for breast milk:
  BPA was found in cord blood, maternal blood, breast milk and peritoneal fluid collected simultaneously from the same individuals (n = 9-21) in a range of 0.21-0.79 ppb. The correlation factors between BPA concentrations of different samples were relatively high: r = 0.626 for maternal blood-cord blood, 0.755 for cord blood-breast milk, and 0.785 for serum-peritoneal fluid from infertile patients.1)
• Procedure according to the Analysis Guideline: detection limit 0.5 ppb:
  Freshly collected samples of cord blood, maternal blood, breast milk and peritoneal fluid from 221 cases were analyzed by the procedure according to the Analysis Guideline. BPA in blood was found in 2 cases only, the maximum being 1.7 ppb. BPA was not found in maternal blood, cord blood and peritoneal fluid.2)
• HPLC-couloarray method:
  Bisphenol A conjugate in the urine of 80 women (26-43 years of age) with a chief complaint of infertility was determined by HPLC-couloarray. The median creatinine concentration was 2.2 μmol/g. Most of bisphenol A in urine was in the conjugated form. The somewhat higher value than the LC-MS results is believed to be inherent to the method.3)

• Hexachlorobenzene was found in 100% of general and maternal peripheral blood samples and 88% of cord blood samples in a range of 0.03-0.10 ppb. The hexachlorobenzene levels in the peripheral blood and the peritoneal fluid obtained from the same individuals had a significant positive correlation (rank correlation factor 0.722 for n = 12, p = 0.017).1)

• Methyl 4-hydroxybenzoate was found in cord blood and breast milk, which had probably received the compound from the blood of the mothers exposed during pregnancy.1)

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