Advisory Committee on Health Effects of Endocrine Disruptors
The Supplement II to the Intermediary Report
1.2.3.1

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• Glassware: Heat to 450℃ for at least five hours in an oven.
• Rotary evaporator: Prevent contamination from ambient air by fitting an active carbon column and air filter to the stopcock open to air, or by providing a trap ball.
• Gas blower for concentrating sample extracts: Provide a portion filled with active carbon or Florisil in the gas line.

3.2.2. Reagents
1) Use products of high purity grade (for precision analysis, dioxin analysis, residual pesticides and PCB analysis, phthalic acid ester analysis, HPLC grade, etc.).
2) Purify the reagents as necessary by distillation, heating or cleaning.
3) Confirm that the quantities used in the procedures described in Part 2 do not affect the quantitative results.
Some examples of reagent purification are given below:

• Purified water: Wash water from a water purifier with n-hexane as necessary.
• Sulfuric acid: Use products of high purity, e.g. semiconductor grade. Wash with n-hexane as necessary.
• Anhydrous sodium sulfate: Use products of high purity, e.g. for residual pesticide analysis. Heat to 450℃ for at least five hours as necessary.
• Silica gel: Wash silica gel for column chromatography ultrasonically in methanol, dry under a reduced pressure, and heat to 130℃ for about 18 hours in a glass beaker. Transfer the gel into a sealable container and store in a desiccator.
• Active carbon-silica gel: Wash active carbon-silica gel ultrasonically in acetone, filtrate, and purify by Soxhlet extraction with toluene. Dry the purified gel in a rotary evaporator under a reduced pressure, transfer into a sealable container, and store in a desiccator.

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