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Advisory Committee on Health Effects of Endocrine Disruptors
The Supplement II to the Intermediary Report
1.2.3.2 |
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[Recent topics]
The U.S. EPA has set a reference dose (RfD) of bisphenol A of
0.05 mg/kg/day based on chronic toxicity experiments on rats. A
draft BPA risk assessment by EU in 2002 proposes a tolerable
daily intake of 0.01 mg/kg/day, which was obtained by
multiplying a NOAEL of 5 mg/kg/day, established by chronic
toxicity tests on rats, by a factor of uncertainty of 1/500.
The HPLC has generally been used for tests of BPA-containing
polycarbonate utensils and determination of BPA released from
them. Analysis of BPA in blood, urine and other biological
samples, however, need a method of far higher sensitivity,
selectivity and reliability due to BPA concentrations far lower
than in plastics. Recent reports on BPA in biological samples
generally use the GC/MS or LC/MS(/MS). Capillary columns used in
GC have high resolution. The GC/CS with negative CI, with its
high sensitivity and selectivity, has been adopted in several
studies for BPA analysis in biological samples. BPA should,
however, be derivatized to prevent its phenol groups from being
adsorbed to the column and causing tailing of the peak. The
LC/MS is, on the other hand,
does not need derivatization and has high sensitivity and
selectivity. It is therefore the most popular method for
analysis of BPA in biological samples. |
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