|
4 Guideline for determination of endocrine disruptors in
feeds for experimental animals Part 1: General testing
1. Introduction
Endocrine disruptors can be present in feeds for experimental
animals. The concentrations of endocrine disruptors in feeds are
generally very low. In addition to requirements on analytical
instruments and environment, a certain level of skills in the
analytical procedure is indispensable to obtain reliable
results. The present guideline summarizes general precautions
for determination of endocrine disruptors in feeds for
experimental animals.
Procedures other than described here may be used insofar as the
reliability of the results is ensured.
2. Acquisition, transport and storage of samples
1) Do not allow the sample to contact with PVC gloves. Use latex
gloves if necessary.
2) When using gloves, perform a blank test to confirm that they
do not contaminate the sample.
3) Use sampling devices made of stainless steel or glass after
confirming that they do not contaminate the sample.
4) Use sample containers made of glass or fluorinated resins.
Perform a blank test beforehand to confirm that the containers
do not contaminate the sample.
5) Obtain an amount of sample that allows at least two
analytical runs and divide into two lots for repetition. To
assure the uniformity of the sample, at least 1 kg is desirable.
6) Store the sample in a cold, dark place.
7) Use containers made of glass or fluorinated resins for
transportation and storage of the sample. Perform a blank test
beforehand to confirm that the containers do not contaminate the
sample.
|