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Advisory Committee on Health Effects of Endocrine Disruptors
The Supplement II to the Intermediary Report
1.2.6 |
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Analysis of feed water was conducted for 11 combinations of
bottles and caps obtained from four suppliers. Considering the
actual mode of use of the bottle, 100 ml pure water was analyzed
out of 200 ml filled in the bottle, either after letting stand
for 24 h at ambient temperature, or after sterilizing for 20 min
at 121 and cooled down. Of the samples left at ambient
temperature, five showed 0.03-0.11 ng/ml NP. All the sterilized
samples except one presented 0.03-0.63 ng/ml NP. These
concentrations correspond to daily NP intake of 0.24-5.04 ng for
a mouse (assuming a daily water intake of 8 ml) and 1.35-28.35
ng for a rat (daily water intake 45 ml).
Contamination in the autoclave was checked by placing purified
water in the beaker (not closed) and analyzed before and after
sterilization. No NP was detected (determination limit 0.02 ng/ml).
(d) Phytoestrogens in feeds
Most of the feed samples studied (37 out of 40) contained
phytoestrogens. The highest concentration, 569 Ęg/g, corresponds
to a daily phytoestrogen intake of 3,414 Ęg for a mouse
(assuming a daily feed intake of 6 g) and 14,225 ng for a rat
(daily feed intake 25 g).
Phytoestrogen was not detected (< 0.2 Ęg/g) in commercially
available "phytoestrogen-free" feeds.
(e) 17 Ŕ-Estradiol in feeds
17 Ŕ-Estradiol (estradiol, E2) level was under the determination
limit (0.50 ng/g) for 20 feeds samples except one product, which
contained 0.52 } 0.03 ng/g E2. These feeds are for mice and
rats. The analytical value corresponds to a daily E2 intake of
3.1 ng for a mouse (daily feed intake 6 g) and 13 ng for a rat
(daily feed intake 25 g).
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