| |
|
|
 |
|
Advisory Committee on Health Effects of Endocrine Disruptors
The Supplement II to the Intermediary Report
1.2.4.2 |
| |
contents
Detailed contents
<< prev
next >> |
| |
Analysis of 4-nonylphenol in animal feeds
[Outline]
Animal feeds are pretreated by an essential oil distillator and
analyzed by a liquid chromatograph/mass spectrometer
(LC/MS(/MS))1
qualitatively and quantitatively.
This procedure is also applicable to litter and water feed.
[Reagents]
Confirm by chromatography that all the reagents present no
problem in NP analysis.
(i) Standard NP: 4- Nonylphenol of the environmental analysis
grade (mixed2,
≥95.0%)
(ii) Preparation of standard solutions: Place 100 mg standard NP
in a 100 ml measuring flask, and add hexane to 100 ml. Dilute
with methanol as appropriate.
(iii) Organic solvents: Residual pesticide analysis,
environmental analysis or LC-MS grade.
(iv) Purified water: Milli-Q water or commercial distilled
water.
(v) Internal standard: 4-(1-Methyl)octylphenol-d5 (m-OP-d5)3.
An absolute calibration curve may be used if recovery ratio or
reproducibility presents no difficulty.
[Tools]
Confirm beforehand that the tools used for sample preparation do
not contain NP.
Wash the tools always in the specified conditions. Heat
glassware to 200℃ for at least 2 h and let cool down in a place
where no NP contamination from the environment is expected. Wash
with acetone immediately before use.
Place 100 ml water and 3 ml hexane in the essential oil
distillator, and distill for about 30 min. Repeat this operation
several times4.
Discard he hexane layer after every distillation run.
|
|
contents
Detailed contents
<< prev
next >> |
|
|
|
