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Last updated date: March 30, 2015
 

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Advisory Committee on Health Effects of Endocrine Disruptors
The Supplement II to the Intermediary Report
1.2.4.2

 

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  Analysis of 4-nonylphenol in animal feeds
[Outline]

Animal feeds are pretreated by an essential oil distillator and analyzed by a liquid chromatograph/mass spectrometer (LC/MS(/MS))1 qualitatively and quantitatively.
This procedure is also applicable to litter and water feed.

[Reagents]
Confirm by chromatography that all the reagents present no problem in NP analysis.
(i) Standard NP: 4- Nonylphenol of the environmental analysis grade (mixed2, 95.0%)
(ii) Preparation of standard solutions: Place 100 mg standard NP in a 100 ml measuring flask, and add hexane to 100 ml. Dilute with methanol as appropriate.
(iii) Organic solvents: Residual pesticide analysis, environmental analysis or LC-MS grade.
(iv) Purified water: Milli-Q water or commercial distilled water.
(v) Internal standard: 4-(1-Methyl)octylphenol-d5 (m-OP-d5)3. An absolute calibration curve may be used if recovery ratio or reproducibility presents no difficulty.

[Tools]
Confirm beforehand that the tools used for sample preparation do not contain NP.
Wash the tools always in the specified conditions. Heat glassware to 200℃ for at least 2 h and let cool down in a place where no NP contamination from the environment is expected. Wash with acetone immediately before use.
Place 100 ml water and 3 ml hexane in the essential oil distillator, and distill for about 30 min. Repeat this operation several times4. Discard he hexane layer after every distillation run.
 

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