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Transgenic mouse "gpt delta" homepage


Protocol (gpt assay, Spi- assay)
Sequence
Publication List



Last update in 2011


Protocol(revision log)

2008〜2011年 TGラット共同研究用プロトコル(gptアッセイ, Spi-アッセイ)
6tg selection protocol4.02ja.doc(gptアッセイ プロトコル 日本語版)
gpt assay protocol 4.11en.doc(gptアッセイ プロトコル 英語版)
Protocol spi modified 2.11en.doc(Spi-アッセイ プロトコル 英語版)
packaging efficiency.doc(パッケージング効率測定用プロトコル 日本語版)
gptdelta data sheet form.xls(gptアッセイ, Spi-アッセイ 記入用データシート)
MS-word, excelファイルです。マウスを右クリックして「対象をファイルに保存」でダウンロードしてください。

Transpack Packaging Extract マニュアルPDF(Agilent Technologies, Inc.(旧Stratagene社))
RecoverEase(tm) DNA Isolation Kit マニュアルPDF(Agilent Technologies, Inc.(旧Stratagene社))

gpt Assay

Sequencing of gpt gene

Spi- Assay

Preparation of packaging extract for lambda phage in vitro packaging


Sequence
  • gpt gene of E. coli {ecogpt(coding459bp).txt} [GenBank ACCESSION X00221 K01784]
  • red/gam gene of lambda phage {gam-redBA.txt, gam-redBA(aa).txt} [GenBank ACCESSION J02459]
  • Tentative sequence of lambda EG10 DNA {eg10v20.txt}

  • Publication List (~2011)

    Title
    Author
    Journal

    Comprehensive toxicity study of safrole using a medium-term animal model with gpt delta rats.
    Jin M, Kijima A, Suzuki Y, Hibi D, Inoue T, Ishii Y, Nohmi T, Nishikawa A, Ogawa K, Umemura T.
    Toxicology. 2011;290(2-3):313-322.

    Modulatory effects of capsaicin on N-diethylnitrosamine (DEN)-induced mutagenesis in Salmonella typhimurium YG7108 and DEN-induced hepatocarcinogenesis in gpt delta transgenic rats.
    Toyoda-Hokaiwado N, Yasui Y, Takamune M, Yamada M, Muramatsu M, Masumura K, Ohta T, Tanaka T. Nohmi T.
    Genes and Environment, 2011;33(4):160-166.

    Site-specific in vivo mutagenicity in the kidney of gpt delta rats given a carcinogenic dose of ochratoxin A.
    Hibi D, Suzuki Y, Ishii Y, Jin M, Watanabe M, Sugita-Konishi Y, Yanai T, Nohmi T, Nishikawa A, Umemura T.
    Toxicol Sci. 2011;122(2):406-414.

    Aflatoxin B1-DNA adduct formation and mutagenicity in livers of neonatal male and female B6C3F1 mice.
    Woo LL, Egner PA, Belanger CL, Wattanawaraporn R, Trudel LJ, Croy RG, Groopman JD, Essigmann JM, Wogan GN.
    Toxicol Sci. 2011;122(1):38-44.

    Chemopreventive effects of silymarin against 1,2-dimethylhydrazene plus dextran sodium sulfate-induced inflammation-associated carcinogenicity and genotoxicitiy in the colon of gpt delta rats.
    Toyoda-Hokaiwado N, Yasui Y, Muramatsu M, Masumura K, Takamune M, Yamada M, Ohta T, Tanaka T, Nohmi T.
    Carcinogenesis, 2011;32(10):1512-1517.

    Involvement of mismatch repair proteins in adaptive responses induced by N-methyl-N'-nitro-N-nitrosoguanidine against γ-induced genotoxicity in human cells.
    Yamamoto A, Sakamoto Y, Masumura K, Honma M, Nohmi T.
    Mutat Res. 2011;713(1-2):56-63.

    Acrylamide genotoxicity in young versus adult gpt delta male rats.
    Koyama N, Yasui M, Kimura A, Takami S, Suzuki T, Masumura K, Nohmi T, Masuda S, Kinae N, Matsuda T, Imai T, Honma M.
    Mutagenesis. 2011;26(4):545-549.

    Antigenotoxic effects of p53 on spontaneous and ultraviolet light B-induced deletions in the epidermis of gpt delta transgenic mice.
    Masumura K, Sakamoto Y, Ikeda M, Asami Y, Tsukamoto T, Ikehata H, Kuroiwa Y, Umemura T, Nishikawa A, Tatematsu M, Ono T, Nohmi T.
    Environ Mol Mutagen. 2011;52(3):244-52.

    Effects of co-treatment of dextran sulfate sodium and MeIQx on genotoxicity and possible carcinogenicity in the colon of p53-deficient mice.
    Okamura T, Ishii Y, Suzuki Y, Inoue T, Tasaki M, Kodama Y, Nohmi T, Mitsumori K, Umemura T, Nishikawa A.
    J Toxicol Sci. 2010;35(5):731-41.

    Enhancing effects of carbon tetrachloride on in vivo mutagenicity in the liver of mice fed 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx).
    Okamura T, Ishii Y, Suzuki Y, Inoue T, Tasaki M, Kodama Y, Nohmi T, Mitsumori K, Umemura T, Nishikawa A.
    J Toxicol Sci. 2010;35(5):709-20.

    Mutagenic potency of Helicobacter pylori in the gastric mucosa of mice is determined by sex and duration of infection.
    Sheh A, Lee CW, Masumura K, Rickman BH, Nohmi T, Wogan GN, Fox JG, Schauer DB.
    Proc Natl Acad Sci U S A. 2010;107(34):15217-22.

    Oxidative DNA damage and reporter gene mutation in the livers of gpt delta rats given non-genotoxic hepatocarcinogens with cytochrome P450-inducible potency.
    Tasaki M, Umemura T, Suzuki Y, Hibi D, Inoue T, Okamura T, Ishii Y, Maruyama S, Nohmi T, Nishikawa A.
    Cancer Sci. 2010;101(12):2525-30.

    Radiation dose-rate effect on mutation induction in spleen and liver of gpt delta mice.
    Okudaira N, Uehara Y, Fujikawa K, Kagawa N, Ootsuyama A, Norimura T, Saeki K, Nohmi T, Masumura K, Matsumoto T, Oghiso Y, Tanaka K, Ichinohe K, Nakamura S, Tanaka S, Ono T.
    Radiat Res. 2010;173(2):138-47.

    Integration of in vivo genotoxicity and short-term carcinogenicity assays using F344 gpt delta transgenic rats: in vivo mutagenicity of 2,4-diaminotoluene and 2,6-diaminotoluene structural isomers.
    Toyoda-Hokaiwado N, Inoue T, Masumura K, Hayashi H, Kawamura Y, Kurata Y, Takamune M, Yamada M, Sanada H, Umemura T, Nishikawa A, Nohmi T.
    Toxicol Sci. 2010;114(1):71-8.

    Spontaneous and induced gpt and Spi- mutant frequencies in gpt delta transgenic rodents
    Kenichi Masumura
    Genes and Environ. 2009;31:105-118.

    Genotoxicity of nano/microparticles in in vitro micronuclei, in vivo comet and mutation assay systems.
    Totsuka Y, Higuchi T, Imai T, Nishikawa A, Nohmi T, Kato T, Masuda S, Kinae N, Hiyoshi K, Ogo S, Kawanishi M, Yagi T, Ichinose T, Fukumori N, Watanabe M, Sugimura T, Wakabayashi K.
    Part Fibre Toxicol. 2009;6:23.

    Role of Parp-1 in suppressing spontaneous deletion mutation in the liver and brain of mice at adolescence and advanced age.
    Shibata A, Maeda D, Ogino H, Tsutsumi M, Nohmi T, Nakagama H, Sugimura T, Teraoka H, Masutani M.
    Mutat Res. 2009; 664(1-2):20-7.

    Genotoxic responses to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells.
    Xu A, Chai Y, Nohmi T, Hei TK.
    Part Fibre Toxicol. 2009;6:3.

    In vivo mutagenesis caused by diesel exhaust in the testis of gpt delta transgenic mice
    Hashimoto H A, Amanuma K, Masumura K, Nohmi T, Aoki Y.
    Genes and Environment 2009; 31(1):1-8.

    Spontaneous mutagenesis in rodents: spontaneous gene mutations identified by neutral reporter genes in gpt delta transgenic mice and rats
    Masumura K and Nohmi T
    Journal of Health Science 2009; 55(1):40-49.

    Possible participation of oxidative stress in causation of cell proliferation and in vivo mutagenicity in kidneys of gpt delta rats treated with potassium bromate.
    Umemura T, Tasaki M, Kijima A, Okamura T, Inoue T, Ishii Y, Suzuki Y, Masui N, Nohmi T, Nishikawa A.
    Toxicology. 2009; 257(1-2):46-52.

    Differential effects of low- and high-dose X-rays on N-ethyl-N-nitrosourea-induced mutagenesis in thymocytes of B6C3F1 gpt-delta mice.
    Yamauchi K, Kakinuma S, Sudo S, Kito S, Ohta Y, Nohmi T, Masumura KI, Nishimura M, Shimada Y.
    Mutat Res. 2008; 640(1-2):27-37.

    Mutation Spectra in Cisplatin- and Transplatin-treated GDL1 Cells Clarified the Different Mode of Action of These Compounds in Mammalian Cells
    Takeiri A, Mishima M, Tanaka K, Shioda A, Harada A, Masumura K, Nohmi T.
    Genes and Environment 2007; 29(3):89-99.

    Detection of oxidative DNA damage, cell proliferation and in vivo mutagenicity induced by dicyclanil, a non-genotoxic carcinogen, using gpt delta mice.
    Umemura T, Kuroiwa Y, Tasaki M, Okamura T, Ishii Y, Kodama Y, Nohmi T, Mitsumori K, Nishikawa A, Hirose M.
    Mutat Res. 2007; 633(1):46-54.

    Mutations in the lungs of gpt delta transgenic mice following inhalation of diesel exhaust.
    Hashimoto AH, Amanuma K, Hiyoshi K, Sugawara Y, Goto S, Yanagisawa R, Takano H, Masumura K, Nohmi T, Aoki Y.
    Environ Mol Mutagen. 2007;48(8):682-93.

    Enhanced spontaneous and benzo(a)pyrene-induced mutations in the lung of Nrf2-deficient gpt delta mice.
    Aoki Y, Hashimoto AH, Amanuma K, Matsumoto M, Hiyoshi K, Takano H, Masumura K, Itoh K, Nohmi T, Yamamoto M.
    Cancer Res. 2007;67(12):5643-8.

    New insight into intrachromosomal deletions induced by chrysotile in the gpt delta transgenic mutation assay.
    Xu A, Smilenov LB, He P, Masumura K, Nohmi T, Yu Z, Hei TK.
    Environ Health Perspect. 2007;115(1):87-92.

    Lack of in vivo mutagenicity and oxidative DNA damage by flumequine in the livers of gpt delta mice.
    Kuroiwa Y, Umemura T, Nishikawa A, Kanki K, Ishii Y, Kodama Y, Masumura K, Nohmi T, Hirose M.
    Arch Toxicol. 2007;81(1):63-9.

    Combined genotoxic effects of radiation and a tobacco-specific nitrosamine in the lung of gpt delta transgenic mice.
    Ikeda M, Masumura K, Sakamoto Y, Wang B, Nenoi M, Sakuma K, Hayata I, Nohmi T.
    Mutat Res. 2007;626(1-2):15-25.

    Chemopreventive Effects of Nobiletin against Genotoxicity Induced by 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in the Lung of gpt delta Transgenic Mice.
    Ikeda M, Masumura K, Matsui K, Kohno H, Sakuma K, Tanaka T, Nohmi T.
    Genes and Environment 2006;28(3):84-91.

    Deletion and single nucleotide substitution at G:C in the kidney of gpt delta transgenic mice after ferric nitrilotriacetate treatment.
    Jiang L, Zhong Y, Akatsuka S, Liu YT, Dutta KK, Lee WH, Onuki J, Masumura K, Nohmi T, Toyokuni S.
    Cancer Sci. 2006;97(11):1159-67.

    In vivo mutagenicity and initiation following oxidative DNA lesion in the kidneys of rats given potassium bromate.
    Umemura T, Kanki K, Kuroiwa Y, Ishii Y, Okano K, Nohmi T, Nishikawa A, Hirose M.
    Cancer Sci. 2006;97(9):829-35.

    A newly established GDL1 cell line from gpt delta mice well reflects the in vivo mutation spectra induced by mitomycin C.
    Takeiri A, Mishima M, Tanaka K, Shioda A, Harada A, Watanabe K, Masumura K, Nohmi T.
    Mutat Res. 2006;609(1):102-15.

    In vivo mutagenesis in the lungs of gpt-delta transgenic mice treated intratracheally with 1,6-dinitropyrene.
    Hashimoto AH, Amanuma K, Hiyoshi K, Takano H, Masumura K, Nohmi T, Aoki Y.
    Environ Mol Mutagen. 2006;47(4):277-83.

    IL-10 deficiency leads to somatic mutations in a model of IBD.
    Sato Y, Takahashi S, Kinouchi Y, Shiraki M, Endo K, Matsumura Y, Kakuta Y, Tosa M, Motida A, Abe H, Imai G, Yokoyama H, Nomura E, Negoro K, Takagi S, Aihara H, Masumura KI, Nohmi T, Shimosegawa T.
    Carcinogenesis 2006;27(5):1068-73

    MX, a by-product of water chlorination, lacks in vivo genotoxicity in gpt delta mice but inhibits gap junctional intercellular communication in rat WB cells.
    Nishikawa A, Sai K, Okazaki K, Son HY, Kanki K, Nakajima M, Kinae N, Nohmi T, Trosko JE, Inoue T, Hirose M.
    Environ Mol Mutagen. 2006;47(1):48-55.

    Mechanisms of chemopreventive effects of 8-methoxypsoralen against 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced mouse lung adenomas.
    Miyazaki M, Yamazaki H, Takeuchi H, Saoo K, Yokohira M, Masumura KI, Nohmi T, Funae Y, Imaida K, Kamataki T.
    Carcinogenesis 2005;26(11):1947-55

    Molecular nature of intrachromosomal deletions and base substitutions induced by environmental mutagens.
    Nohmi T, Masumura K.
    Environ Mol Mutagen. 2005;45(2-3):150-61.

    In vivo mutagenesis induced by benzo[a]pyrene instilled into the lung of gpt delta transgenic mice.
    Hashimoto AH, Amanuma K, Hiyoshi K, Takano H, Masumura KI, Nohmi T, Aoki Y.
    Environ Mol Mutagen. 2005;45(4):365-73.

    Parp-1 deficiency causes an increase of deletion mutations and insertions/rearrangements in vivo after treatment with an alkylating agent.
    Shibata A, Kamada N, Masumura KI, Nohmi T, Kobayashi S, Teraoka H, Nakagama H, Sugimura T, Suzuki H, Masutani M.
    Oncogene. 2005;24(8):1328-1337

    In vivo mutational analysis of liver DNA in gpt delta transgenic rats treated with the hepatocarcinogens N-nitrosopyrrolidine, 2-amino-3-methylimidazo[4,5-f]quinoline, and di(2-ethylhexyl)phthalate.
    Kanki K, Nishikawa A, Masumura KI, Umemura T, Imazawa T, Kitamura Y, Nohmi T, Hirose M.
    Mol Carcinog. 2005 Jan;42(1):9-17.

    gpt delta transgenic mouse: a novel approach for molecular dissection of deletion mutations in vivo.
    Nohmi T and Masumura K.
    Adv Biophys. 2004;38:97-121.

    Efficient method for mapping and characterizing structures of deletion mutations in gpt delta mice using Southern blot analysis with oligo DNA probes.
    Shibata A, Masutani M, Kamada N, Masumura KI, Nakagama H, Kobayashi S, Teraoka H, Suzuki H, Nohmi T.
    Environ Mol Mutagen. 2004;43(3):204-207.

    Low dose genotoxicity of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) in gpt delta transgenic mice.
    Masumura K, Horiguchi M, Nishikawa A, Umemura T, Kanki K, Kanke Y, Nohmi T.
    Mutat Res. 2003;541(1-2):91-102.

    Effect of atm disruption on spontaneously arising and radiation-induced deletion mutations in mouse liver.
    Furuno-Fukushi I, Masumura K, Furuse T, Noda Y, Takahagi M, Saito T, Hoki Y, Suzuki H, Wynshaw-Boris A, Nohmi T, Tatsumi K.
    Radiat Res. 2003;160(5):549-58.

    In vivo transgenic mutation assays.
    Thybaud V, Dean S, Nohmi T, de Boer J, Douglas GR, Glickman BW, Gorelick NJ, Heddle JA, Heflich RH, Lambert I, Martus HJ, Mirsalis JC, Suzuki T, Yajima N.
    Mutat Res. 2003;540(2):141-51.

    Potent genotoxicity of aminophenylnorharman, formed from non-mutagenic norharman and aniline, in the liver of gpt delta transgenic mouse.
    Masumura K, Totsuka Y, Wakabayashi K, Nohmi T.
    Carcinogenesis 2003;24(12):1985-93. Epub 2003 Sep 11.

    Improvement of the Spi- assay for mutations in gpt delta mice by including magnesium ions during plaque formation.
    Shibata A, Masutani M, Nozaki T, Kamada N, Fujihara H, Masumura K, Nakagama H, Sugimura T, Kobayashi S, Suzuki H, Nohmi T.
    Environ Mol Mutagen 2003;41(5):370-372

    Novel transgenic rat for in vivo genotoxicity assays using 6-thioguanine and Spi- selection.
    Hayashi H, Kondo H, Masumura K, Shindo Y, Nohmi T.
    Environ Mol Mutagen 2003;41(4):253-9

    Molecular characterization of mitomycin C-induced large deletions and tandem-base substitutions in the bone marrow of gpt delta transgenic mice.
    Takeiri A, Mishima M, Tanaka K, Shioda A, Ueda O, Suzuki H, Inoue M, Masumura K, Nohmi T.
    Chem Res Toxicol. 2003;16(2):171-179.

    High accumulation of oxidative DNA damage, 8-hydroxyguanine, in Mmh/Ogg1 deficient mice by chronic oxidative stress.
    Arai T, Kelly VP, Minowa O, Noda T, Nishimura S.
    Carcinogenesis 2002;23(12):2005-10.

    Heavy-ion-induced mutations in the gpt delta transgenic mouse: Comparison of mutation spectra induced by heavy-ion, X-ray, and gamma-ray radiation.
    Masumura K, Kuniya K, Kurobe T, Fukuoka M, Yatagai F, Nohmi T.
    Environ Mol Mutagen 2002;40(3):207-15

    Heavy-ion-induced mutations in the gpt delta transgenic mouse: Effect of p53 gene knockout.
    Yatagai F, Kurobe T, Nohmi T, Masumura K, Tsukada T, Yamaguchi H, Kasai-Eguchi K, Fukunishi N.
    Environ Mol Mutagen 2002;40(3):216-25

    Mutation induction by heavy-ion irradiation of gpt delta transgenic mice.
    Yatagai F, Nohmi T, Kusakabe M, Masumura K, Yoshiki A, Yamaguchi H, Kurobe T, Kuniya K, Hanaoka F, Yano Y.
    Phys Med. 2001;17 Suppl 1:192-3.

    Further characterization and validation of gpt delta transgenic mice for quantifying somatic mutations in vivo.
    Swiger RR, Cosentino L, Masumura KI, Nohmi T, Heddle JA.
    Environ Mol Mutagen. 2001;37(4):297-303.

    Reporter gene transgenic mice as a tool for analyzing the molecular mechanisms underlying experimental carcinogenesis.
    Nishikawa A, Suzuki T, Masumura K, Furukawa F, Miyauchi M, Nakamura H, Son HY, Nohmi T, Hayashi M, Hirose M.
    J Exp Clin Cancer Res 2001;20(1):111-5

    Molecular Nature of Ultraviolet B Light-induced Deletions in the Murine Epidermis
    Horiguchi M, Masumura K, Ikehata H, Ono T, Kanke Y, Nohmi T
    Cancer Res 2001;61:3913-3918.

    UVB-induced mutations in the skin of gpt delta transgenic mouse: a novel transgenic mouse mutagenesis assay
    Horiguchi M, Masumura K, Nohmi T
    Env Sci 2000;7:249-258

    Recent advances in the protocols of transgenic mouse mutation assays.
    Nohmi T, Suzuki T, Masumura K
    Mutat Res 2000;455(1-2):191-215

    Characterization of mutations induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in the colon of gpt delta transgenic mouse: novel G:C deletions beside runs of identical bases.
    Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Wakabayashi K, Nohmi T
    Carcinogenesis 2000;21(11):2049-2056

    In vivo transgenic mutation assays.
    Heddle JA, Dean S, Nohmi T, Boerrigter M, Casciano D, Douglas GR, Glickman BW, Gorelick NJ, Mirsalis JC, Martus HJ, Skopek TR, Thybaud V, Tindall KR, Yajima N
    Environ Mol Mutagen. 2000;35(3):253-9.

    [Development of novel genotoxicity assays by genetic engineering methods]
    Nohmi T.
    Kokuritsu Iyakuhin Shokuhin Eisei Kenkyusho Hokoku 2000;(118):1-20

    トランスジェニックマウスgpt deltaを用いた点突然変異と欠失突然変異の検出
    能美健彦, 増村健一
    環境変異原研究, 22: 85-90 (2000)

    Mmh/Ogg1 gene inactivation results in accumulation of 8-hydroxyguanine in mice.
    Minowa O, Arai T, Hirano M, Monden Y, Nakai S, Fukuda M, Itoh M, Takano H, Hippou Y, Aburatani H, Masumura K, Nohmi T, Nishimura S, Noda T.
    Proc Natl Acad Sci USA. 2000;97(8):4156-4161.

    Efficient detection of deletions induced by a single treatment of mitomycin C in transgenic mouse gpt delta using the Spi(-) selection.
    Okada N, Masumura K, Nohmi T, Yajima N
    Environ Mol Mutagen. 1999;34(2-3):106-11.

    UVB-induced gpt mutations in the skin of gpt delta transgenic mice.
    Horiguchi M, Masumura K, Ikehata H, Ono T, Kanke Y, Sofuni T, Nohmi T.
    Environ Mol Mutagen. 1999;34(2-3):72-9.

    Mutagenicity of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in the new gpt delta transgenic mouse.
    Masumura K, Matsui K, Yamada M, Horiguchi M, Ishida K, Watanabe M, Ueda O, Suzuki H, Kanke Y, Tindall KR, Wakabayashi K, Sofuni T, Nohmi T.
    Cancer Lett. 1999 Sep 1;143(2):241-4.

    Spi(-) selection: An efficient method to detect gamma-ray-induced deletions in transgenic mice.
    Nohmi T, Suzuki M, Masumura K, Yamada M, Matsui K, Ueda O, Suzuki H, Katoh M, Ikeda H, Sofuni T.
    Environ Mol Mutagen. 1999;34(1):9-15.

    Spectra of gpt mutations in ethylnitrosourea-treated and untreated transgenic mice.
    Masumura K, Matsui M, Katoh M, Horiya N, Ueda O, Tanabe H, Yamada M, Suzuki H, Sofuni T, Nohmi T.
    Environ Mol Mutagen. 1999;34(1):1-8.

    A new transgenic mouse mutagenesis test system using Spi- and 6-thioguanine selections.
    Nohmi T, Katoh M, Suzuki H, Matsui M, Yamada M, Watanabe M, Suzuki M, Horiya N, Ueda O, Shibuya T, Ikeda H, Sofuni T.
    Environ Mol Mutagen. 1996;28(4):465-70.




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    masumura@nihs.go.jp