Research Achievements

研究業績

再生・細胞医療製品部の研究業績を、原著論文・総説・単行本に分けて年度別に掲載しています。
（なお、原著論文については著者の内、研究部在籍者（発表当時の在籍者を含む）を下線で示しています。）

2026 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015

2026

2件
原著論文 新着

Multi-site validation and applicability assessment of a practical 3D Colony formation assay for detecting malignantly transformed Cells

Bando K, Kusakawa S (co-first author), Adachi H, Yamamoto M, Bando Y, Fujiwara R, Iwata M, Kitanaka A, Nishioka Y, Ogimura E, Enoki S, Terai O, Yoda T, Yotsumoto T, Zaizen K, Yasuda S, Sato Y

Cytotherapy, 2026, in press.

DOI
原著論文

Development of a digital analysis system for a novel 3D culture-based colony formation to detect malignantly transformed cells in human cell-based therapeutic products

Kusakawa S, Yang T, Sawada R, Kato R, Sato Y, Yasuda S

Regen Ther, 2026, 31:101052.

DOI

2025

2件
原著論文

Increasing robustness of in vitro assay for immnosuppressive effect of mesenchymal stromal/stem cells: The role of inflammatory cytokine production by peripheral blood mononuclear cells

Sawada R, Kusakawa S, Kusuhara M, Tanaka K, Miura T, Yasuda S, Sato Y

Regen Ther, 2025, 28:321-332.

DOI
原著論文

Using qPCR and ddPCR to study biodistribution of cell therapy products: a multi-site evaluation

Fujita E, Yamamoto S, Hanada T, Jogasaki S, Koga Y, Yatsuda Y, Kakizaki Y, Jo Y, Asano Y, Yonezawa K, Moriya Y, Nakayama M, Arimura Y, Okawa Y, Komatsu H, Ito M, Suzuki S, Kuroda T, Yasuda S, Kamiyama Y, Sato Y

Cytotherapy, 2025, 27(1):51-65.

DOI

2024

4件
原著論文

Detection of residual pluripotent stem cells in cell therapy products utilizing droplet digital PCR: an international multisite evaluation study

Yasuda S, Bando K, Henry MP, Libertini S, Watanabe T, Bando H, Chen C, Fujimori K, Harada K, Kuroda T, Limmens M, Marginean D, Moss D, Pereira Mouriès L, Nicholas NS, Smart MJK, Terai O, Sato Y

Stem Cells Transl Med, 2024, szae058.

DOI
原著論文

Multisite studies for optimization of a highly efficient culture assay used for in vitro detection of residual undifferentiated human pluripotent stem cells intermingled in cell therapy products

Watanabe T, Yasuda S, Kusakawa S, Kuroda T, Furukawa H, Futamura M, Shimizu S, Morishita A, Hata S, Koeda A, Komatsu K, Sato Y

Regen Ther, 2024, 21:26:315-323.

DOI
原著論文

Protocol improvement and multisite validation of a digital soft agar colony formation assay for tumorigenic transformed cells intermingled in cell therapy products

Bando K, Kusakawa S (co-first author), Adachi H, Yamamoto M, Iwata M, Kitanaka A, Ogimura E, Osada T, Tamura M, Terai O, Watanabe T, Yoda T, Yotsumoto T, Zaizen K, Sato Y

Cytotherapy, 2024, 26(7):769-777.

DOI
原著論文

ROR2 expression predicts human induced pluripotent stem cell differentiation into neural stem/progenitor cells and GABAergic neurons

Kuroda T, Yasuda S, Matsuyama S, Miura T, Sawada R, Matsuyama A, Yamamoto Y, Morioka MS, Kawaji H, Kasukawa T, Itoh M, Akutsu H, Kawai J, Sato Y

Sci Rep, 2024;14(1):690.

DOI

2023

3件
原著論文

Evaluation of next-generation sequencing performance for in vitro detection of viruses in biological products

Hirai T, Kataoka K, Yuan Y, Yusa K, Sato Y, Uchida K, Kono K

Biologicals, 2023;85:101739.

DOI
原著論文

Single-Cell RNA-Seq Reveals LRRC75A-Expressing Cell Population Involved in VEGF Secretion of Multipotent Mesenchymal Stromal/Stem Cells Under Ischemia

Miura T, Kouno T (co-first author), Takano M, Kuroda T, Yamamoto Y, Kusakawa S, Morioka MS, Sugawara T, Hirai T, Yasuda S, Sawada R, Matsuyama S, Kawaji H, Kasukawa T, Itoh M, Matsuyama A, Shin JW, Umezawa A, Kawai J, Sato Y

Stem Cells Transl Med, 2023;12(6):379-390.

DOI
原著論文

International evaluation study of a highly efficient culture assay for detection of residual human pluripotent stem cells in cell therapies

Watanabe T, Yasuda S, Chen CL, Delsing L, Fellows MD, Foldes G, Kusakawa S, Mouries LP, Sato Y

Regen Med, 2023;18(3):219-227.

DOI

2022

5件
原著論文

Evaluation of the reproducibility and positive controls of cellular immortality test for the detection of immortalized cellular impurities in human cell-processed therapeutic products

Hirai T, Kono K, Kusakawa S, Yasuda S, Sawada R, Morishita A, Hata S, Wakita A, Kageyama T, Takahashi R, Watanabe S, Shiraishi N, Sato Y

Regen Ther, 2022;21:540-546.

DOI
原著論文

Attitude and perception survey for the Japanese pharmaceutical industry to utilize next-generation sequencing for virus safety assessment of biologics

Hirasawa R, Takakura M, Hirai T, Kono K, Sato Y

Translat Regulat, 2022;4:61-67.

DOI
原著論文

Myocardial TRPC6-mediated Zn2+ influx induces beneficial positive inotropy through β-adrenoceptors

Oda S, Nishiyama K, Furumoto Y, Yamaguchi Y, Nishimura A, Tang X, Kato Y, Numaga-Tomita T, Kaneko T, Mangmool S, Kuroda T, Okubo R, Sanbo M, Hirabayashi M, Sato Y, Nakagawa Y, Kuwahara K, Nagata R, Iribe G, Mori Y, Nishida M

Nat Commun, 2022;13:6374.

DOI
原著論文

A single mutation in the E2 glycoprotein of hepatitis C virus broadens the claudin specificity for its infection

Shirasago Y, Fukazawa H, Nagase S, Shimizu Y, Mizukami T, Wakita T, Suzuki T, Tani H, Kondoh M, Kuroda T, Yasuda S, Sato Y, Hanada K, Fukasawa M

Sci Rep, 2022;24:20243.

DOI
原著論文

Correlation Between Genetic Abnormalities in Induced Pluripotent Stem Cell-Derivatives and Abnormal Tissue Formation in Tumorigenicity Tests

Yamamoto T, Sato Y, Yasuda S, Shikamura M, Tamura T, Takenaka C, Takasu N, Nomura M, Dohi H, Takahashi M, Mandai M, Kanemura Ym, Nakamura M, Okano H, Kawamata S

Stem Cells Transl Med, 2022;11:527-538.

DOI

2021

6件
原著論文

Deep learning-based in vitro detection method for cellular impurities in human cell-processed therapeutic products

Matsuzaka Y, Kusakawa S, Uesawa Y, Sato Y, Sato M

Appl Sci, 2021;11(20):9755.

DOI
原著論文

Biodistribution studies for cell therapy products: Current status and issues

Kamiyama Y, Naritomo Y, Moriya Y, Yamamoto S, Kitahashi T, Maekawa T, Yahara M, Hanada T, Uchiyama A, Noumaru A, Koga Y, Higuchi T, Ito M, Komatsu H, Miyoshi S, Kimura S, Umeda N, Fujita E, Tanaka N, Sugita T, Takayama S, Kurogi A, Yasuda S, Sato Y

Regen Ther, 2021;18:202-216.

DOI
原著論文

A selective cytotoxic adenovirus vector for concentration of pluripotent stem cells in human pluripotent stem cell-derived neural progenitor cells

Hirai T, Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Koizumi N, Utoguchi N, Mizuguchi H, Sato Y

Sci Rep, 2021;11(1):11407.

DOI
原著論文

Infectivity assessment of porcine endogenous retrovirus using high-throughput sequencing technologies

Kono K, Kataoka K, Yuan Y, Yusa K, Uchida K, Sato Y

Biologicals, 2021;71:1-8.

DOI
原著論文

A simple method to estimate the in-house limit of detection for genetic mutations with low allele frequencies in whole-exome sequencing analysis by next-generation sequencing

Miura T, Yasuda S, Sato Y

BMC Genomic Data, 2021;22:8.

DOI
原著論文

Multisite studies for validation and improvement of a highly efficient culture (HEC) assay for detection of undifferentiated human pluripotent stem cells intermingled in cell therapy products

Watanabe T, Yasuda S, Kusakawa S, Kuroda T, Futamura M, Ogawa M, Mochizuki H, Kikkawa E, Furukawa H, Nagaoka M, Sato Y

Cytotherapy, 2021;23(2):176-183.

DOI

2020

2件
原著論文

A highly sensitive method for the detection of recombinant PERV-A/C env RNA using next generation sequencing technologies

Kono K, Kataoka K, Yuan Y, Yusa K, Uchida K, Sato Y

Sci Rep, 2020;10:21935.

DOI
原著論文

Research and development strategy for future embryonic stem cell-based therapy in Japan

Umezawa A, Sato Y, Kusakawa S, Amagase R, Akutsu H, Nakamura K, Kasahara M, Matsubara Y, Igarashi T

JMA Journal, 2020; DOI: 10.31662/jmaj.2018-0029.

DOI

2019

4件
原著論文

CXCL4/PF4 is a predictive biomarker of cardiac differentiation potential of human induced pluripotent stem cells

Ohashi F, Miyagawa S, Yasuda S, Miura T, Kuroda T, Itoh M, Kawaji H, Ito E, Yoshida S, Saito A, Sameshima T, Kawai J, Sawa Y, Sato Y

Sci Rep, 2019;9:4638.

DOI
原著論文

Development of selective cytotoxic viral vectors for concentration of undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells

Kono K, Sawada R, Kuroda T, Yasuda S, Matsuyama S, Matsuyama A, Mizuguchi H, Sato Y

Sci Rep, 2019 Mar 6;9(1):3630.

DOI
原著論文

Tumorigenicity assay essential for facilitating safety studies of hiPSC-derived cardiomyocytes for clinical application

Ito E, Miyagawa S, Takeda M, Kawamura A, Harada A, Iseoka H, Yajima S, Sougawa N, Mochizuki-Oda N, Yasuda S, Sato Y, Sawa Y

Sci Rep, 2019;9:1881.

DOI
原著論文

SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation

Kuroda T, Yasuda S, Tachi S, Matsuyama S, Kusakawa S, Tano K, Miura T, Matsuyama A, Sato Y

Nat Commun, 2019;10:2175.

DOI

2018

3件
原著論文

Hypoxic stress-induced interaction of filamin/actin cytoskeleton with Drp1 causes mitochondrial hyperfission-associated myocardial senescence

Nishimura A, Shimauchi T, Tanaka T, Shimoda K, Toyama T, Kitajima N, Ishikawa T, Shindo N, Numaga-Tomita T, Yasuda S, Sato Y, Kuwahara K, Kumagai Y, Akaike T, Ide T, Ojida A, Mori Y, Nishida M

Sci Signal, 2018;11:eaat5185.

DOI
原著論文

Tumorigenicity-associated characteristics of human iPS cell lines

Yasuda S, Kusakawa S, Kuroda T, Miura T, Tano K, Takada N, Matsuyama S, Matsuyama A, Nasu M, Umezawa A, Hayakawa T, Tsutsumi H, Sato Y

PLOS ONE, 2018;13:e0205022.

DOI
原著論文

A roundtable on responsible innovation with autologous stem cells in Australia, Japan and Singapore

Lysaght T, Munsie M, Castricum A, Hui JHP, Okada K, Sato Y, Sawa Y, Stewart C, Tan LK, Tan LHY, Sugii S

Cytotherapy, 2018;20(9):1103-1109.

DOI

2017

1件
原著論文

Identification of a Gene Encoding Slow Skeletal Muscle Troponin T as a Novel Marker for Immortalization of Retinal Pigment Epithelial Cells

Kuroda T, Yasuda S, Nakashima H, Takada N, Matsuyama S, Kusakawa S, Umezawa A, Matsuyama A, Kawamata S, Sato Y

Sci Rep, 2017;7:8163-8174.

DOI

2016

6件
原著論文

TRPC3-GEF-H1 axis mediates pressure overload-induced cardiac fibrosis

Numaga-Tomita T, Kitajima N, Kuroda T, Nishimura A, Miyano K, Yasuda S, Kuwahara K, Sato Y, Ide T, Birnbaumer L, Sumimoto H, Mori Y, Nishida M

Sci Rep, 2016;6:39383.

DOI
原著論文

TRPC3 positively regulates reactive oxygen species driving maladaptive cardiac remodeling

Kitajima N, Numaga-Tomita T, Watanabe M, Kuroda T, Nishimura A, Miyano K, Yasuda S, Kuwahara K, Sato Y, Ide T, Birnbaumer L, Sumimoto H, Mori Y, Nishida M

Sci Rep, 2016;6:37001.

DOI
原著論文

Report of the International Regulatory Forum on Human Cell Therapy and Gene Therapy Products

Hayakawa T, Harris I, Joung J, Kanai N, Kawamata S, Kellathur S, Koga J, Lin YC, Maruyama Y, McBlane J, Nishimura T, Renner M, Ridgway A, Salmikangas P, Sakamoto N, Sato D, Sato Y, Toda Y, Umezawa A, Werner M, Wicks S

Biologicals, 2016;44(5):467-479.

DOI
原著論文

Application of cell growth analysis to the quality assessment of human cell-processed therapeutic products as a testing method for immortalized cellular impurities

Hasebe-Takada N, Kono K (co-first author), Yasuda S, Sawada R, Matsuyama A, Sato Y

Regen Ther, 2016;5:49-54.

DOI
原著論文

In Vitro endothelialization test of biomaterials using immortalized endothelial cells

Kono K, Hiruma H, Kobayashi S, Sato Y, Tanaka M, Sawada R, Niimi S

PLoS ONE, 2016;11(6):e0158289.

DOI
原著論文

Glutamine oxidation is indispensable for survival of human pluripotent stem cells

Tohyama S, Fujita J, Hishiki T, Matsuura T, Hattori F, Ohno R, Kanazawa H, Seki T, Nakajima K, Kishino Y, Okada M, Hirano A, Kuroda T, Yasuda S, Sato Y, Yuasa S, Sano M, Suematsu M, Fukuda K

Cell Metab, 2016;23:663-674.

DOI

2015

10件
原著論文

Separate cellular localizations of human T-lymphotropic virus 1 (HTLV-1) Env and glucose transporter type 1 (GLUT1) are required for HTLV-1 Env-mediated fusion and infection

Maeda Y, Terasawa H, Tanaka Y, Mitsuura C, Nakashima K, Yusa K, Harada S

J Virol, 2015;89:502-511.

DOI
原著論文

Ultra-sensitive detection of tumorigenic cellular impurities in human cell-processed therapeutic products by digital analysis of soft agar colony formation

Kusakawa S, Yasuda S, Kuroda T, Kawamata S, Sato Y

Sci Rep, 2015;5:17892.

DOI
原著論文

Highly sensitive droplet digital PCR method for detection of residual undifferentiated cells in cardiomyocytes derived from human pluripotent stem cells

Kuroda T, Yasuda S, Matsuyama S, Tano K, Kusakawa S, Sawa Y, Kawamata S, Sato Y

Regen Ther, 2015;2:17-23.

DOI
原著論文

A study on ensuring the quality and safety of pharmaceuticals and medical devices derived from the processing of autologous human somatic stem cells

Hayakawa T, Aoi T, Umezawa A, Ozawa K, Sato Y, Sawa Y, Matsuyama A, Yamanaka S, Yamato M

Regen Ther, 2015;2:57-69.

DOI
原著論文

A study on ensuring the quality and safety of pharmaceuticals and medical devices derived from the processing of allogeneic human somatic stem cells

Hayakawa T, Aoi T, Umezawa A, Ozawa K, Sato Y, Sawa Y, Matsuyama A, Yamanaka S, Yamato M

Regen Ther, 2015;2:70-80.

DOI
原著論文

A study on ensuring the quality and safety of pharmaceuticals and medical devices derived from processing of autologous human induced pluripotent stem(-like) cells

Hayakawa T, Aoi T, Umezawa A, Ozawa K, Sato Y, Sawa Y, Matsuyama A, Yamanaka S, Yamato M

Regen Ther, 2015;2:81-94.

DOI
原著論文

A study on ensuring the quality and safety of pharmaceuticals and medical devices derived from processing of allogeneic human induced pluripotent stem(-Like) cells

Hayakawa T, Aoi T, Umezawa A, Ozawa K, Sato Y, Sawa Y, Matsuyama A, Yamanaka S, Yamato M

Regen Ther, 2015;2:95-108.

DOI
原著論文

A study on ensuring the quality and safety of pharmaceuticals and medical devices derived from the processing of human embryonic stem cells

Hayakawa T, Aoi T, Umezawa A, Ozawa K, Sato Y, Sawa Y, Matsuyama A, Yamanaka S, Yamato M

Regen Ther, 2015;2:109-122.

DOI
原著論文

Fluorescent Image Analysis of HIV-1 and HIV-2 Uncoating Kinetics in the Presence of Old World Monky TRIM5α

Takeda E, Kono K, Hulme AE, Hope TJ, Nakayama EE, Shioda T

PLoS ONE, 2015;10:e0121199.

DOI
原著論文

Characterization of the cell growth analysis for detection of immortal cellular impurities in human mesenchymal stem cells

Kono K, Takada N (co-first author), Yasuda S, Sawada R, Niimi S, Matsuyama A, Sato Y

Biologicals, 2015;43:146-149.

DOI