NIHS 国立医薬品食品衛生研究所 National Institute of Health Sciences
生化学部 Division of Biochemistry

 

安全性承認済み遺伝子組換え食品の検査法
 
安全性審査済みの組換えDNA技術応用食品の検査方法について(平成24年11月16日消食表第201号)
 
(別添)安全性審査済みの組換えDNA技術応用食品の検査方法

crop plant method detection of probe & primer name sequence amplicon (bp) standard plasmid or control sample
Soybean quantitative Real-time PCR Le1 Le1n 02-5’ Le1-n02 5’-GCCCTCTACTCCACCCCCA-3’ 118    
(kernel) (endogenous gene) Le1n 02-3’ 5’-GCCCATCTG CAAGCCTTTTT-3’  
      Le1-Taq   5’-FAM-AGCTTCGCCGCTTCCTTCAACTTCAC-TAMRA-3’      
  quantitative Real-time PCR RRS RRS 01-5’ RRS-01 5’-CCTTTAGGATTTCAGCATCAGTGG-3’ 121 GM Soybean(RRS) Detection Plasmid Set-ColE1/TE-
    RRS 01-3’ 5’-GACTTGTCGCCGGGAATG-3’ GM Soybean(RRS) Detection Plasmid Set-ColE1/TE-
      RRS-Taq   5’-FAM-CGCAACCGCCCGCAAATCC-TAMRA-3’      
  quantitative Real-time PCR LLS KVM175 KVM175 & SMO001 5’-GCAAAAAAGCGGTTAGCTCCT-3’ 64 GM Soybean(LLS) Detection Plasmid Set-ColE1/TE-
    SMO001 5’-ATTCAGGCTGCGCAACTGTT-3’ GM Soybean(LLS) Detection Plasmid Set-ColE1/TE-
      TM031   5’-FAM-CGGTCCTCCGATCGCCCTTCC-TAMRA-3’      
  quantitative Real-time PCR RRS2 MON89788-F MON89788-F & MON89788-R 5’-TCCCGCTCTAGCGCTTCAAT-3’ 139 GM Soybean(RR2) Detection Plasmid Set-ColE1/TE-
  MON89788-R 5’-TCGAGCAGGACCTGCAGAA-3’ GM Soybean(RR2) Detection Plasmid Set-ColE1/TE-
      MON89788-P   5’-FAM-CTGAAGGCGGGAAACGACAATCTG-TAMRA-3’      
Soybean qualitative Real-time PCR Le1 Le1n 02-5’ Le1-n02 5’-GCCCTCTACTCCACCCCCA-3’ 118  
(processed food) (endogenous gene) Le1n 02-3’ 5’-GCCCATCTG CAAGCCTTTTT-3’  
      Le1-Taq   5’-FAM-AGCTTCGCCGCTTCCTTCAACTTCAC-TAMRA-3’      
  qualitative Real-time PCR P35S P35S 1-5’ P35S-1 5’-ATTGATGTGATATCTCCACTGACGT-3’ 101  
  P35S 1-3’ 5’- CCTCTCCAAATGAAATGAACTTCCT-3’  
      P35S-Taq   5’-FAM-CCCACTATCCTTCGCAAGACCCTTCCT-TAMRA-3’      
  qualitative Real-time PCR RRS2 MON89788-F MON89788-F & MON89788-R 5’-TCCCGCTCTAGCGCTTCAAT-3’ 139  
  MON89788-R 5’-TCGAGCAGGACCTGCAGAA-3’  
      MON89788-P   5’-FAM-CTGAAGGCGGGAAACGACAATCTG-TAMRA-3’      
Maize quantitative Real-time PCR SSIIb SSIIb 3-5’ SSIIb-3 5’-CCAATCCTTTGACATCTGCTCC-3’ 114    
(kernel: screening1)   (endogenous gene) SSIIb 3-3’ 5’-GATCAGCTTTGGGTCCGGA-3’  
      SSIIb-Taq   5’-FAM-AGCAAAGTCAGAGCGCTGCAATGCA-TAMRA-3’      
  quantitative Real-time PCR P35S P35S 1-5’ P35S-1 5’-ATTGATGTGATATCTCCACTGACGT-3’ 101  
    P35S 1-3’ 5’- CCTCTCCAAATGAAATGAACTTCCT-3’  
      P35S-Taq   5’-FAM-CCCACTATCCTTCGCAAGACCCTTCCT-TAMRA-3’      
  quantitative Real-time PCR GA21 GA21-3-5’ GA21-3 5’-GAAGCCTCGGCAACGTCA-3’ 133  
    GA21-3-3’ 5’-ATCCGGTTGGAAAGCGACTT-3’  
      GA21-Taq   5’-FAM-AAGGATCCGGTGCATGGCCG-TAMRA-3’      
  quantitative Real-time PCR MIR604 MIR604 primer F MIR604-1 5’-GCGCACGCAATTCAACAG-3’ 76  
    MIR604 primer R 5’-GGTCATAACGTGACTCCCTTAATTCT-3’  
      MIR604 probe   5’-FAM-AGGCGGGAAACGACAATCTGATCATG-TAMRA-3’      
  quantitative Real-time PCR MIR162 MIR162-f1 MIR162-1 5’-GCGCGGTGTCATCTATGTTACTAG-3’ 92  
    MIR162-r1 5’-TGCCTTATCTGTTGCCTTCAGA-3’  
      MIR162-p1   5’-FAM-TCTAGACAATTCAGTACATTAAAAACGTCCGCCA-TAMRA-3’      
Maize multiplex Real-time PCR SSIIb SSIIb 3-5’ SSIIb-3 5’-CCAATCCTTTGACATCTGCTCC-3’ 114   Maize GMO Standard ERM-BF413gk (10% MON810)
(kernel: screening2)   (endogenous gene) SSIIb 3-3’ 5’-GATCAGCTTTGGGTCCGGA-3’ Maize GMO Standard ERM-BF413ak (Blank MON810)
(individual kernel)     SSIIb-TaqV   5’-VIC-AGCAAAGTCAGAGCGCTGCAATGCA-TAMRA-3’      
(processed food) multiplex Real-time PCR P35S P35S 1-5’ P35S-1 5’-ATTGATGTGATATCTCCACTGACGT-3’ 101  
    P35S 1-3’ 5’- CCTCTCCAAATGAAATGAACTTCCT-3’  
      P35S-Taq   5’-FAM-CCCACTATCCTTCGCAAGACCCTTCCT-TAMRA-3’      
  multiplex Real-time PCR TNOS NOS ter 3-5’ 5’-GCATGTAATAATTAACATGTAATGCATGAC-3’ 110  
  NOS ter 2-3’ 5’-CGCTATATTTTGTTTTCTATCGCGT-3’  
      NOS-Taq   5’-FAM-AGATGGGTTTTTATGATTAGAGTCCCGCAA-TAMRA-3’      
Maize multiplex Real-time PCR SSIIb SSIIb 3-5’ SSIIb-3 5’-CCAATCCTTTGACATCTGCTCC-3’ 114    
(group testing)   (endogenous gene) SSIIb 3-3’ 5’-GATCAGCTTTGGGTCCGGA-3’  
      SSIIb-TaqHB   5’-HEX-AGCAAAGTCAGAGCGCTGCAATGCA-BHQ1-3’      
  multiplex Real-time PCR P35S P35S 1-5’ P35S-1 5’-ATTGATGTGATATCTCCACTGACGT-3’ 101  
    P35S 1-3’ 5’- CCTCTCCAAATGAAATGAACTTCCT-3’  
      P35S-TaqFB   5’-FAM-CCCACTATCCTTCGCAAGACCCTTCCT-BHQ1-3’      
  multiplex Real-time PCR TNOS NOS ter 2-5’ NOS ter-2 5'-GTCTTGCGATGATTATCATATAATTTCTG-3' 151  
    NOS ter 2-3’ 5’-CGCTATATTTTGTTTTCTATCGCGT-3’  
      NOS-TaqFB   5’-FAM-AGATGGGTTTTTATGATTAGAGTCCCGCAA-BHQ1-3’      
  multiplex Real-time PCR IPC IPC 1-5’ IPC-1 5'-CCGAGCTTACAAGGCAGGTT-3' 100 Internal Positive Control
    IPC 1-3’ 5'-TGGCTCGTACACCAGCATACTAG-3'  
      IPC-TaqFB   5'-TAGCTTCAAGCATCTGGCTGTCGGC-3'      
Maize real-time PCR array SSIIb SSIIb 3-5’ SSIIb-3 5’-CCAATCCTTTGACATCTGCTCC-3’ 114 GM Maize Detection Positive Control Plasmid
(determination of GM event)   (endogenous gene) SSIIb 3-3’ 5’-GATCAGCTTTGGGTCCGGA-3’ GM Maize Detection Positive Control Plasmid
    SSIIb-Taq   5’-FAM-AGCAAAGTCAGAGCGCTGCAATGCA-TAMRA-3’      
  real-time PCR array Bt11 Bt11 3-5’ 5’-AAAAGACCACAACAAGCCGC-3’ 127 GM Maize Detection Positive Control Plasmid
    Bt11 3-3’ 5’-CAATGCGTTCTCCACCAAGTACT-3’ GM Maize Detection Positive Control Plasmid
      Bt11-2-Taq   5’-FAM-CGACCATGGACAACAACCCAAACATCA-TAMRA-3’      
  real-time PCR array Event176 E176 2-5’ 5’-TGTTCACCAGCAGCAACCAG-3’ 100 GM Maize Detection Positive Control Plasmid
    E176 2-3’ 5’-ACTCCACTTTGTGCAGAACAGATCT-3’ GM Maize Detection Positive Control Plasmid
      E176-Taq   5’-FAM-CCGACGTGACCGACTACCACATCGA-TAMRA-3’      
  real-time PCR array GA21 GA21 3-5’ GA21-3 5’-GAAGCCTCGGCAACGTCA-3’ 133 GM Maize Detection Positive Control Plasmid
    GA21 3-3’ 5’-ATCCGGTTGGAAAGCGACTT-3’ GM Maize Detection Positive Control Plasmid
      GA21-2-Taq   5’-FAM-AAGGATCCGGTGCATGGCCG-TAMRA-3’      
  real-time PCR array MON810 M810 2-5’ 5’-GATGCCTTCTCCCTAGTGTTGA-3’ 113 GM Maize Detection Positive Control Plasmid
    M810 2-3’ 5’-GGATGCACTCGTTGATGTTTG-3’ GM Maize Detection Positive Control Plasmid
      M810-Taq   5’-FAM-AGATACCAAGCGGCCATGGACAACAA-TAMRA-3’      
  real-time PCR array MON863 M863 1-5’ 5’-TGACCCTACTTGTTCGGATGG-3’ 111 Maize GMO Standard ERM-BF416d (10% MON863)
    M863 1-3’ 5’-GCATTTGTAGGTGCCACCTTC-3’  
      MON863-Taq   5’-FAM-CACCCCAAAGTGTACCAAGCTTTCCGA-TAMRA-3’      
  real-time PCR array NK603 NK603 1-5’ 5’-GGCCAGCAAGCCTTGTAGC-3’ 113 Maize GMO Standard ERM-BF415f (5% NK603)
    NK603 1-3’ 5’-ATCCCGACTCTCTTCTCAAGCATA-3’  
      NK603-Taq   5’-FAM-ATGACCTCGAGTAAGCTTGTTAACGCGGC-TAMRA-3’      
  real-time PCR array T25 PM1 5’-TCAATTGCCCTTTGGTCTTCTGA-3’ 155 Maize Bayer CropScience Event T25 Leaf DNA 0306-H6
    revPM1 5’-TACGACATGATACTCCTTCCAC-3’  
      FBP3   5’-FAM-TCATTGAGTCGTTCCGCCATTGTCG-TAMRA-3’      
  real-time PCR array TC1507 TC1507 1-5’ 5’-TGAGTTGATTCCAGTTACTGCCA-3’ 111 Maize GMO Standard ERM-BF418d (10% 1507)
    TC1507 1-3’ 5’-ATGTTAGTCGCAACGAAACCG-3’  
      TC1507-Taq   5’-FAM-ACTCGAGTAAGGATCCGTCGACCTGCAG-TAMRA-3’      
  real-time PCR array MIR604 MIR604 primer F MIR604-1 5’-GCGCACGCAATTCAACAG-3’ 76 Maize GMO Standard ERM-BF423d (10% MIR604)
    MIR604 primer R 5’-GGTCATAACGTGACTCCCTTAATTCT-3’ Maize Syngenta Event MIR604 Powder 0607-A2 
      MIR604 probe   5’-FAM-AGGCGGGAAACGACAATCTGATCATG-TAMRA-3’      
  real-time PCR array MON88017 M88017 1-5’ 5’-ATCGTGTGACAACGCTAGCA-3’ 150 Maize Monsanto Company Event MON88017 Powder 0406-D
    M88017 1-3’ 5’-CATATTGACCATCATACTCATTGCT-3’  
      M88017-1-Taq   5’-FAM-TGCCGGAGTATGACGGTGACGATATATTCA-TAMRA-3’      
  real-time PCR array DAS-59122-7 DAS59122-7-rb1f 5’-GGGATAAGCAAGTAAAAGCGCTC-3’ 86 Maize GMO Standard ERM-BF424 (0, 0.1, 1, 10% 59122)
    DAS59122-7-rb1r 5’-CCTTAATTCTCCGCTCATGATCAG-3’  
      DAS59122-7-rb1s probe 5’-FAM-TTTAAACTGAAGGCGGGAAACGACAA-TAMRA-3’      
  real-time PCR array MON89034 MON89034 primer1 5’-TTCTCCATATTGACCATCATACTCATT-3’ 77 Maize Monsanto Company Event MON89034 Powder 0906-E
    MON89034 primer2 5’-CGGTATCTATAATACCGTGGTTTTTAAA-3’  
      MON89034 probe   5’-FAM-ATCCCCGGAAATTATGTT-MGBNFQ-3'      
  real-time PCR array MIR162 MIR162-f1 MIR162-1 5’-GCGCGGTGTCATCTATGTTACTAG-3’ 92 Maize Syngenta Event MIR 162 Powder 1208-A
  MIR162-r1 5’-TGCCTTATCTGTTGCCTTCAGA-3’  
  MIR162-p1 5’-FAM-TCTAGACAATTCAGTACATTAAAAACGTCCGCCA-TAMRA-3’  
Papaya qualitative Real-time PCR CHY Q-Chy-1F2 Q-Chy-1F2 &
Q-Chy-2R
5’-CCATGCGATCCTCCCA-3’ 72    
    Q-Chy-2R 5’-CATCGTAGCCATTGTAACACTAGCTAA-3’  
      Q-Chy-P(new)   5’-FAM-TTCCCTTCATCCATTCCCACTCTTGAGA-TAMRA-3’      
  qualitative Real-time PCR PRSV-cp (55-1) PRSV-cp F PRSV-cp F &
PRSV-cp R
5’-CAGCCTTAGATGCTTCAAGAAAAGA-3’ 71 GM Papaya Detection 55-1 Positive Control Plasmid 2
    PRSV-cp R 5’-TCCGCCTCCATCCAGTCTATT-3’ GM Papaya Detection 55-1 Positive Control Plasmid 2
      PRSV-cp P   5’-FAM-TCTTCTAGCTTCCCGGCAACAAT-TAMRA-3’      

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