NIHS 国立医薬品食品衛生研究所 National Institute of Health Sciences
生化学部 Division of Biochemistry

 

安全性未承認遺伝子組換え食品(作物)の検査法

Crop Plant Method Gene discription Transgene or Target gene Detection of Forward primer Reverse primer Probe amplicon (bp)
Rice qualitative Real-time PCR endogenous gene (phospholipase D) PLD2 5’-GCTTAGGGAACAGGGAAGTAAAGTT-3’ 5’-CTTAGCATAGTCTGTGCCATCCA-3’ 5’-FAM-TGAGTATGAACCTGCAGGTCGC-TAMRA-3’ 80  
  qualitative Real-time PCR insect-resistant cry1Ac Bt63 5’-GCAGGAGTGATTATCGACAGATTC-3’ 5’-AAGACCGGCAACAGGATTCA-3’ 5’-FAM-AATAAGTCGAGGTACCGAGCTCGAATTTCCC-TAMRA-3’ 147  
  qualitative Real-time PCR insect-resistant cry1Ab/Ac NNBt 5’-GCAGGAGTGATTATCGACAGATTC-3’ 5’-AAGACCGGCAACAGGATTCA-3’ 5’-FAM-AATGAGAATTCGGTACCCCGACCTGCA-TAMRA-3’ 147  
  qualitative Real-time PCR insect-resistant cowpea trypsin inhibitor CpTI2 5’- TGCAAGTCCAGGGATGAAGAT-3’ 5’-ACCGGCAACAGGATTCAATC-3’ 5’-FAM-ATGAGAAAGATGAACTCTAG-MGB-3’ 121  
     
Rice qualitative Real-time PCR endogenous gene (phospholipase D) PLD 5’-TGGTGAGCGTTTTGCAGTCT-3’ 5’-CTGATCCACTAGCAGGAGGTCC-3’ 5’-VIC-TGTTGTGCTGCCAATGTGGCCTG-TAMRA-3’ 68  
  qualitative Real-time PCR herbicide-tolerant  phosphinothricin N-acetyltransferase  (S. hygroscopicus) LL601 5’-TATCCTTCGCAAGACCCTTCC-3’ 5’-ATGTCGGCCGGGCGTCGTTCTG-3’ 5’-FAM-TCTATATAAGGAAGTTCATTTCATT-MGB-3’ 136  
                   
Wheat qualitative Real-time PCR endogenous gene (proline-rich protein) PRP 5’-GCACCCATGATGAGTACTACTATTCTGTA-3’ 5’-TGCAAACGAATAAAAGCATGTG-3’ 5’-FAM-CTGTGCACATGACTCAGTTGTTCTTTCGTG-TAMRA-3’ 117  
  qualitative Real-time PCR
(event-specific)
herbicide-tolerant  cp4epsps MON71800 5’-TTCTTCTCTCTCTTTGAATCTCAATACAA-3’ 5’-CCCCCATTTGGACGTGAA-3’ 5’-FAM-TCCCCCTCTCTAATTC-MGB-3’ 95  
  qualitative Real-time PCR
(construct-specific)
herbicide-tolerant  cp4epsps MON71800/MON71700 5’-CAA GCA TAT GAA TGA CCT CGA GTA AG-3’ 5’-CGT GTC TAC ATT CAC GTC CAA ATG-3’ 5’-FAM-AAA CTT CAC GAT CGA TGC G-MGB-3’    
Flax qualitative Real-time PCR endogenous gene (stearoyl-acyl carrier protein desaturase 2) SAD 5’-GCTCAACCCAGTCACCACCT-3’ 5’-TGCGAGGAGATCTGGAGGAG-3’ 5’-FAM-TGTTGAGGGAGCGTGTTGAAGGGA-BHQ1-3’ 68  
  qualitative Real-time PCR herbicide-tolerant  mutant acetolactate synthase FP967 5’-AGCGCGCAAACTAGGATAAA-3’ 5’-ACCTTCCGGCTCGATGTCTA-3’ 5’-FAM- CGCGCGCGGTGTCATCTATG-BHQ1-3’ 105  
                   
Papaya qualitative Real-time PCR endogenous gene (chymopapain) CHY 5’-CCATGCGATCCTCCCA-3’ 5’-CATCGTAGCCATTGTAACACTAGCTAA-3’ 5’-FAM-TTCCCTTCATBHQ1CCATTCCCACTCTTGAGA-3’ 73  
  qualitative Real-time PCR promoter Cauliflower mosaic virus 35S promoter CaM 5’-GCCTCTGCCGACAGTGGT -3’ 5’-AAGACGTGGTTGGAACGTCTTC-3’ 5’-FAM-CAAAGATGGACCCCCACCCACG-TAMRA-3’ 82  
  qualitative Real-time PCR virus-resistance Papaya RingSpot Virus coat protein from Taiwan strain PRSV-YK 5’-ACACGGGGGACTCTAGAG -3’ 5’-ACCGGTATCCACAGCTTC-3’ 5’-FAM-TCCCTTCCATGGCGTC-TAMRA-3’ 76  
  qualitative Real-time PCR virus-resistance Papaya RingSpot Virus coat protein from Thailand strain PRSV-SC 5’-CATTTCATTTGGAGAGAACACG-3’ 5’-ACCAGCATCCACAGCTTC-3’ 5’-FAM-ACTCTAGAGGATCCATGTCCAA-TAMRA -3’ 70  
  qualitative Real-time PCR virus-resistance Papaya RingSpot Virus coat protein from Thailand strain PRSV-HN 5’-GAC GAG TAC AAG GAG ACG CC-3’ 5’-GTT GTC ACT GAA GCG GGA AG-3’ 5’-FAM-TGG CTG CTA TTG GGC GAA TCA ACT AC-BHQ1-3’    
Maize conventional PCR endogenous gene (zein) Zein 5’-CCTATAGCTTCCCTTCTTCC-3’ 5’-TGCTGTAATAGGGCTGATGA-3’ 157  
  conventional PCR insect-resistant cry1Ab, cp4epsps Bt10 5’-CACACAGGAGATTATTATAGGGTTACTCA-3’ 5’-ACACGGAAATGTTGAATACTCATACTCT-3’ 117  
  conventional PCR insect-resistant cry1Ab, cp4epsps (for confirmation) Bt10 5’-GCCACAACACCCTCAACCTCA-3’ 5’-GAAGTCGTTGCTCTGAAGAACAT-3’ 151  
  conventional PCR insect-resistant pat, cry9c CBH351 5’-CCTTCGCAAGACCCTTCCTCTATA-3’ 5’-GTAGCTGTCGGTGTAGTCCTCGT-3’ 170  
  conventional PCR insect-resistant pat, cry9c (for confirmation) CBH351 5’-TACTACATCGACCGCATCGA-3’ 5’-CCTAATTCCCTTATCTGGGA-3’ 171  
     
  qualitative Real-time PCR endogenous gene (starch synthase Iib) SSB 5’-CCAATCCTTTGACATCTGCTCC-3’ 5’-GATCAGCTTTGGGTCCGGA-3’ 5’-FAM-AGCAAAAAAAGAGCGCTGCAA-TAMRA-3’ 114  
  qualitative Real-time PCR
 (event-specific)
insect-resistant pat, cry34Ab1, cry35Ab1 DAS59132 5’-CCGCAATGTGTTATTAAGTTGTCTAAG-3’ 5’-GGTGAATGTCGCCGTGTGT-3’ 5’-FAM-CAATTTGTTTACACCAGAGGCCGACACG-TAMRA-3’ unknown  
                   
Rapeseed qualitative Real-time PCR endogenous gene (Brasica rapa acetyl CoA carboxylase) ACCg8 5’-GGTTATATACGGCTTTGTGGTTGC-3’ 5’-AACATCAGGCTGTCCAAGAAAGAT-3’ 5’-VIC-CTATGTCTGAGGAATTATAA-MGB-3’ 78  
  qualitative Real-time PCR endogenous gene (Brasica napus cruciferin) BnC1 5’-GAAGCTCTCCTTCGTGGCTAAA-3’ 5’-TCACGAATTTGAATCTCGATACTCA-3’ 5’-FAM-ACGTGAATCTGATTTTGA-MGB-3’ 75  
  qualitative Real-time PCR endogenous gene  (Aacyl-ACP thioesterase) FatA 5’-GGTCTCTCAGCAAGTGGGTGAT-3’ 5’-TCGTCCCGAACTTCATCTGTAA-3’ 5’-VIC-ATGAACCAAGACACAAGGCGGCTTCA-TAMRA-3’ 76  
  qualitative Real-time PCR herbicide-tolerant  cp4epsps, gox RT73 (cp4 epsps, gox) 5’-CCATATTGACCATCATACTCATTGCT-3’ 5’-GCTTATACGAAGGCAAGAAAAGGA-3’ 5’-FAM-TTCCCGGACATGAAGATCATCCTCCTT-TAMRA-3’ 108  
Potato qualitative Real-time PCR endogenous gene (adenine phosphoribosyl transferase) APRT 5’-TGAAAACGATCCCGATCG-3’ 5’-CAATCCCAGCGATACGTTC-3’ 5’-FAM-TGGCGCCTCATGATCCG-TAMRA-3’    
  qualitative Real-time PCR
(event-specific)
Reduced Black Spot and Acrylamide Event-E12, Asn, Agp, Phl Right border (RB) 5’-TAGTTGGGAGTAGGCTTTATAC-3’ 5’-GATAAAATGGATAAAAACAGATC-3’ 5’-FAM-CGAGTTGGACTACGGTCAGTCACTTTGTACTAG-TAMRA-3’  
  qualitative Real-time PCR
(event-specific)
Reduced Black Spot and Acrylamide Event-F10,  Asn, Agp, Phl Left border (LB) 5’-GAAGCTATAACAATAACTGGTCC-3’ 5’-CACACACTTCGTTTACAC-3’ 5’-FAM-TATATATCCTGCTGGACCAGTTG-TAMRA-3’  
  qualitative Real-time PCR
(event-specific)
Reduced Black Spot and Acrylamide Event-J3,  Asn, Agp, Phl   5’-ATCAAAACCGGTACTCAAATTT-3’ 5’-GAGTTGTCAAATGTGAATTTATTTC-3’ 5’-FAM-CAACAGGACAACCACAAGCTAGGAAACTCAC-TAMRA-3’    

安全性未承認遺伝子組換え食品(動物)の検査法

animal method gene discription inserted gene detection of forward primer reverse primer probe amplicon (bp)
                   
                   

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